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ascl1 tet  (Addgene inc)


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    Addgene inc ascl1 tet
    Figure 3. FOXA2 cooperates with <t>ASCL1</t> in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and
    Ascl1 Tet, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3g+tre3g+ascl1/pm39470735-243-0-7?v=Addgene+inc
    Average 92 stars, based on 4 article reviews
    ascl1 tet - by Bioz Stars, 2026-07
    92/100 stars

    Images

    1) Product Images from "ASCL1 regulates and cooperates with FOXA2 to drive terminal neuroendocrine phenotype in prostate cancer."

    Article Title: ASCL1 regulates and cooperates with FOXA2 to drive terminal neuroendocrine phenotype in prostate cancer.

    Journal: JCI insight

    doi: 10.1172/jci.insight.185952

    Figure 3. FOXA2 cooperates with ASCL1 in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and
    Figure Legend Snippet: Figure 3. FOXA2 cooperates with ASCL1 in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and

    Techniques Used: ChIP-sequencing, Binding Assay, Proximity Ligation Assay, Immunoprecipitation, Expressing



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    Figure 3. FOXA2 cooperates with <t>ASCL1</t> in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and
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    Figure 3. FOXA2 cooperates with <t>ASCL1</t> in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and
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    Figure 3. FOXA2 cooperates with <t>ASCL1</t> in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and
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    Image Search Results


    Figure 3. FOXA2 cooperates with ASCL1 in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and

    Journal: JCI insight

    Article Title: ASCL1 regulates and cooperates with FOXA2 to drive terminal neuroendocrine phenotype in prostate cancer.

    doi: 10.1172/jci.insight.185952

    Figure Lengend Snippet: Figure 3. FOXA2 cooperates with ASCL1 in NEPC. (A) Heatmap of FOXA2 and ASCL1 ChIP-Seq binding intensity in NCI-H660; represents the regions co-occupied by FOXA2 alone or FOXA2 and ASCL1. (B) Proximity ligation assay (PLA) showing ASCL1 and FOXA2 interactions, visualized as red fluorescent dots, indicating molecular proximity (<40 nm), in NCI-H660. Nuclei are counterstained with DAPI (blue). Original magnification, ×20. (C) ASCL1 and FOXA2 interaction was measured by co-immunoprecipitation in NCI-H660. (D) Pathways regulated by ASCL1:FOXA2, shown as –log10 P value using gProfiler. (E) Box plot shows the expression of ASCL1:FOXA2 cobound genes in Adeno, ASCL1-low, and ASCL1-high NEPC patient datasets (3, 9). The middle solid line shows the median. Statistical significance was assessed using 2-tailed unpaired t test, followed by the Bonferroni method (ASCL1-high vs. PRAD P < 2 × 10–16). (F) Box plot shows expression of ASCL1:FOXA2 cobound genes in LuCaP PRAD and NEPC PDXs. The middle solid line shows the median. Statistical significance was assessed using a 2-tailed unpaired t test. (G) Box plot shows expression of ASCL1:FOXA2 cobound genes in 16DCRPC, 42DENZR, 42FENZR, and

    Article Snippet: ASCL1 Tet-on inducible system vectors were pTet-O-ASCL1-T2A-PuroR (Addgene, catalog 162345) and FUW-M2rtTA (Addgene, catalog 20342).

    Techniques: ChIP-sequencing, Binding Assay, Proximity Ligation Assay, Immunoprecipitation, Expressing